| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … The synthetic gene encoding for TcDHFR–TS (purchased from GenScript USA Inc., www.genscript.com), was expressed in E. coli, then protein was harvested and purified, following the experimental procedure described in detail in the Supplementary material (Section S1) … | Get A Quote |
Multi-target approaches are necessary to properly analyze or modify the function of a biochemical pathway or a protein family. An example of such a problem is the repurposing of the known human anti-cancer drugs， antifolates， as selective anti-parasitic agents. This requires considering a set of experimentally validated protein targets in the folate pathway of major pathogenic trypanosomatid parasites and humans: (i) the primary parasite on-targets: pteridine reductase 1 (PTR1) (absent in humans) and bifunctional dihydrofolate reductase-thymidylate synthase (DHFR-TS)， (ii) the primary off-targets: human DHFR and TS， and (iii) the secondary on-target: human folate receptor β， a folate/antifolate trans... More
