The extracellular matrix(ECM)， which is primarily composed of collagens and proteoglycans， plays a key role in cell proliferation， differentiation， and migration and interactions between cells. In this study， we produced chitosan/gelatin/bone marrow stem cells-derived extracellular matrix(C/G/BMSCs-dECM) scaffolds via lyophilization and cross-linking， and chitosan/gelatin(C/G) scaffolds were used as controls. For the C/G/BMSCs-dECM scaffolds， the average pore size was 289.17 ± 80.28 μm; the average porosity was 89.25 ± 3.75%; the average compressive modulus was 0.82 ± 0.07 MPa; and the average water uptake ratio was 13.81 ± 1.00. ， the C/G/BMSCs-dECM scaffolds promoted bone marrow stem cells(BMSCs) attachment and proliferation. Moreover， improved osteogenic differentiation was observed for these scaffolds. Thus， C/G/BMSCs-dECM is a promising material for bone tissue engineering.