Zika virus (ZIKV， genus Flavivirus) has emerged as a major mosquito-transmitted human pathogen， with recent outbreaks associated with an increased incidence of neurological complications， particularly microcephaly and the Guillain-Barré syndrome. Because the virus has only very recently emerged as an important pathogen， research is being hampered by a lack of reliable molecular tools. Here we report an infectious cDNA (icDNA) clone for ZIKV isolate BeH819015 from Brazil， which was selected as representative of South American ZIKV isolated at early stages of the outbreak. icDNA clones were assembled from synthetic DNA fragments corresponding to the consensus sequence of the BeH819015 isolate. Virus rescued from the icDNA clone had properties identical to a natural ZIKV isolate from South America. Variants of the clone-derived virus， expressing nanoluciferase， enhanced green fluorescent or mCherry marker proteins in both mammalian and insect cells and being genetically stable for multiple in vitro passages， were obtained. A ZIKV subgenomic replicon， lacking a prM- and E glycoprotein encoding region and expressing a Gaussia luciferase marker， was constructed and shown to replicate both in mammalian and insect cells. In the presence of the Semliki Forest virus replicon， expressing ZIKV structural proteins， the ZIKV replicon was packaged into virus-replicon particles. Efficient reverse genetic systems， genetically stable marker viruses and packaged replicons offer significant improvements for biological studies of ZIKV infection and disease， as well as for the development of antiviral approaches.