| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | Gene sequence (GenBank accession number AF194963.2) of the C-terminal 34 amino acids truncated 1–3/4-fucosyltransferase from Helicobacter pylori UA948 was codon optimized (host: E. coli) and synthesized with N-terminal NdeI and C-terminal XhoI restriction Enzyme Sites from GenScript (Piscataway, NJ). | Get A Quote |
Helicobacter pylori α1-3/4-fucosyltransferase (Hp3/4FT) was expressed in Escherichia coli at a level of 30 mg L-1 culture and used as a diverse catalyst in a one-pot multienzyme (OPME) system for high-yield production of l-fucose-containing carbohydrates including Lewis antigens such as Lewis a, b, and x, O-sulfated Lewis x, and sialyl Lewis x and human milk fucosides such as 3-fucosyllactose (3-FL), lacto-N-fucopentaose (LNFP) III, and lacto-N-difuco-hexaose (LNDFH) II and III. Noticeably, while difucosylation of tetrasaccharides was readily achieved using an excess amount of donor, the synthesis of LNFP III was achieved by Hp3/4FT-catalyzed selective fucosylation of the N-acetyllactosamine (LacNAc) component... More
