The α Na，K-ATPase (αNKA) is one of four known α isoforms of the mammalian transporter. A deficiency in αNKA is linked to severe movement control disorders. Understanding the pathogenesis of these disorders is limited by an incomplete knowledge of αNKA expression in the brain as well as the challenges associated with identifying living cells that express the isoform for subsequent electrophysiological studies. To address this problem， transgenic mice were generated on the C57BL/6 genetic background， which utilize the mouse α subunit gene (Atp1a3) promoter to drive the expression of ZsGreen1 fluorescent protein. Consistent with published results on αNKA distribution， a ZsGreen1 signal was detected in the brain， but not in the liver， with Atp1a3-ZsGreen1 transgenic mice. The intensity of ZsGreen1 fluorescence in neuronal cell bodies varied considerably in the brain， being highest in the brainstem， deep cerebellar and select thalamic nuclei， and relatively weak in cortical regions. Fluorescence was not detected in astrocytes or white matter areas. ZsGreen1-positive neurons were readily observed in fresh (unfixed) brain sections， which were amenable to patch-clamp recordings. Thus， the αNKA-ZsGreen1 mouse model provides a powerful tool for studying the distribution and functional properties of αNKA-expressing neurons in the brain.