| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | ... 1), the construct contained full length NS2B and N-terminal protease domain of NS3. The amino acid sequence is shown in Fig. 1. The cDNA of NS2B-NS3pro was synthesized by Genscript and cloned into NdeI and XhoI sites of pET29b. ... | Get A Quote |
Dengue protease is a two-component enzyme and is an important drug target against dengue virus. The protease activity and protein stability of dengue nonstructural protein 3 (NS3) require a co-factor region from a four-span membrane protein NS2B. A natural form of dengue protease containing full-length NS2B and NS3 protease domain NS2BFL-NS3pro will be useful for dengue drug discovery. In current study, detergents that can be used for protease purification were tested. Using a water soluble protease construct, 39 detergents were selected for both NS2B and NS2BFL-NS3pro purification. The results showed that 18 detergents were able to sustain the activity of the natural dengue protease and 11 detergents could be ... More
