| ￥3500 | |
| Z05882-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 14 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Serial dilutions of Anti-TIGIT Antibody were added into TIGIT, His, Human, His Tag : Biotinylated CD155, hFc Tag binding reactioins. The half maximal inhibitiory concentration(IC50) is 0.46 μg/ml. »
Immobilized TIGIT, His, Human, His Tag at 1 μg/ml(100 μl/well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 46.9ng/ml determined by ELISA. »
Immobilized TIGIT, His, Human, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. »
The purity of TIGIT, His, Human is greater than 95% as determined by SEC-HPLC. »
TIGIT, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Human CD155, hFc Tag captured on Protein A chip, can bind TIGIT, His, Human, His Tag with an affinity constant of 0.17μM as determined in a SPR assay (Biacore T200). »
| Target Background | TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells. |
| Synonyms | TIGIT; VSIG9; VSTM3; WUCAM |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
