| ￥3000 | |
| Z05839-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 38.1 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Serial dilutions ofSIRP alpha/CD172a, His, Human Ab. were added into Human SIRP alpha V1,Fc Tag: Biotinylated CD47,Fc Tag binding reactions.The half maximal inhibitory concentration(IC50) is 73.46ng/ml. »
Immobilized SIRP alpha/CD172a, His, Human alpha at 0.5 μg/ml(100 μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 95.2ng/ml determined by ELISA. »
The purity of SIRP alpha/CD172a, His, Human is greater than 95% as determined by SEC-HPLC. »
SIRP alpha/CD172a, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
SIRP alpha/CD172a, His, Human, His Tag captured on Protein A Chip via Anti-His Antibody can bind Human CD47, hFc Tag with an affinity constant of 20.6nM as determined in SPR assay (Biacore T200). »
SIRP alpha/CD172a, His, Human Antibody captured on CM5 Chip via Protein A can bind Human Sirpa, His Tag with an affinity constant of 2.81 nM as determined in SPR assay (Biacore T200). »
| Target Background | Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the 'don´t eat me' signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction. |
| Synonyms | CD172a; BIT; MFR; MYD1; MYD-1; P84; PTPNS1; SHPS1; SHPS-1; SIRP alpha; SIRPA; |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
