| ￥2600 | |
| Z05783-100 | |
|
|
|
|
|
|
|
|
|
| Species | Human | ||||
| Protein Construction |
|
||||
| Purity | > 95% as determined by Bis-Tris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 14.95 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 20-25 kDa and 26-32 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Immobilized R spondin 3/RSPO3, His, Human, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. »
Human R spondin 3/RSPO3, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Human LGR-5, hFc Tag captured on CM5 Chip via Protein A can bind R spondin 3/RSPO3, His, Human, His Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200) (Routine Test). »
| Target Background | The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein. |
| Synonyms | R-spondin-3; RSPO3; hPWTSR; hRspo3; PWTSR; THSD2 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
