| ￥2600 | |
| Z05734-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 42.6 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 65-72 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Immobilized PD-1/PDCD1 hFc Chimera, Human, mFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human PD-1, hFc Tag with the EC50 of 90.5ng/ml determined by ELISA. »
Immobilized PD-1/PDCD1 hFc Chimera, Human at 0.5 μg/ml(100 μl/well) on the plate. Dose response curve for Biotinylated Anti-PD1 Antibody, hFc Tag with the EC50 of 16.5ng/ml determined by ELISA. »
The purity of PD-1/PDCD1 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »
PD-1/PDCD1 hFc Chimera, Human on Bis-Tris PAGE under reduced. The purity is greater than 95%. »
PD-1/PDCD1 hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-L2, His-Avi Tag with an affinity constant of 0.344 μM as determined in SPR assay (Biacore T200). »
PD-1/PDCD1 hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-L1, His Tag with an affinity constant of 0.25 µM as determined in SPR assay (Biacore T200). »
| Target Background | Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity. |
| Synonyms | PDCD1; PD1; CD279; SLEB2; PD-1 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
