| ￥3300 | |
| Z05450-100 | |
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| Species | Human | |
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | |
| Endotoxin Level | Less than 0.1EU per μg by the LAL method. | |
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK92 cells. Test result was comparable to standard batch. | |
| Expression System | E.coli | |
| Theoretical Molecular Weight | 18.2 kDa | |
| Apparent Molecular Weight | The protein has a predicted MW of 18.2 kDa same as Bis-Tris PAGE result. | |
| Formulation | Lyophilized from 0.22μm filtered solution in 20mM PB, 250mM NaCl, 50mM L-arginine (pH 7.4). | |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | |
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 1-5 ng/mL (QC Test). »
Immobilized IL-18, Human, No Tag at 5 μg/ml (100μl/well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 62.2ng/ml determined by ELISA. »
The purity of IL-18, Human is greater than 95% as determined by SEC-HPLC. »
IL-18, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
IL-18, Human, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 2.38 nM as determined in SPR assay (Biacore T200). »
| Target Background | Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation. |
| Synonyms | IL-18; IL18; IGIF; IL-1F4; IL-1 gamma; IL1F4; IL-1g; Interleukin 1; IL-1γ; IL-1 γ |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
