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目录产品 » IL-13 hFc Chimera, Human
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IL-13 hFc Chimera, Human

Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).
￥2600
Z05434-100

产品简介
Species Human
Protein Construction
hFc IL-13 (Gly21-Asn132)
Accession # AAK53823
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 39.6 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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图例
IL-13 HFc Chimera, Human

Loaded IL-13 hFc Chimera, Human, hFc Tag on ProA-Biosensor can bind Human IL-13Ra1, His Tag with an affinity constant of 1.15 µM as determined in BLI assay (Gator® Prime). »

IL-13 HFc Chimera, Human

Immobilized Biotinylated Human IL-13Ra1, His Tag at 2 μg/ml (100 μl/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for IL-13 hFc Chimera, Human, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA. »

IL-13 HFc Chimera, Human

Immobilized IL-13 hFc Chimera, Human, hFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 6.8ng/ml determined by ELISA. »

IL-13 HFc Chimera, Human

The purity of IL-13 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »

IL-13 HFc Chimera, Human

IL-13 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

IL-13 HFc Chimera, Human

IL-13 hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-13Ra1, His Tag with an affinity constant of 0.399 μM as determined in SPR assay (Biacore T200). »

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技术背景
Target Background Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).
Synonyms IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; MGC116786; MGC116788; MGC116789
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.