| ￥3500 | |
| Z05397-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Human IFN alpha/beta R1, His Tag captured on CM5 Chip via antihis antibody can bind IFN alpha 1 hFc Chimera, Human in SPR assay (Biacore T200). Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 46.14 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
The purity of IFN alpha 1 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »
IFN alpha 1 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Human IFN alpha/beta R1, His Tag captured on CM5 Chip via anti-his antibody can bind IFN alpha 1 hFc Chimera, Human, hFc Tag with an affinity constant of 0.12 μM as determined in SPR assay (Biacore T200) (QC Test). »
Human IFN alpha/beta R2, His Tag captured on CM5 Chip via anti-his antibody can bind IFN alpha 1 hFc Chimera, Human, hFc Tag with an affinity constant of 11.90 nM as determined in SPR assay (Biacore T200) (QC Test). »
| Target Background | IFN-α, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes. |
| Synonyms | Interferon alpha-1/13;IFN-alpha-1/13;Interferon alpha-D;LeIF D; IFNalpha 1; IFN alpha1;IFNA1;IFN-alpha-1;Interferon alpha 1 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
