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目录产品 » GFRAL/GFR alpha-like hFc Chimera, Human
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GFRAL/GFR alpha-like hFc Chimera, Human

GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses
￥3500
Z05348-100

产品简介
Species Human
Protein Construction
GFRAL/GFR alpha-like (Ser19-Glu351)
Accession # Q6UXV0		 hFc
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 64.6 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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图例
GFRAL/GFR Alpha-like HFc Chimera, Human

Immobilized Human GDF15, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve forGFRAL/GFR alpha-like hFc Chimera, Human, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA. »

GFRAL/GFR Alpha-like HFc Chimera, Human

The purity of  GFRAL/GFR alpha-like hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »

GFRAL/GFR Alpha-like HFc Chimera, Human

GFRAL/GFR alpha-like hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

GFRAL/GFR Alpha-like HFc Chimera, Human

GFRAL/GFR alpha-like hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF15, His Tag with an affinity constant of 0.014 nM as determined in SPR assay (Biacore T200). »

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技术背景
Target Background GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses
Synonyms GFR alpha-like; GFRAL; GRAL; C6orf144
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.