| ￥1500 | |
| Z05259-100 | |
|
|
|
|
|
|
|
|
|
| Species | Human | ||||
| Protein Construction |
|
||||
| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 49 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Immobilized Human EPHB2, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Ephrin-B2/EFNB2 hFc Chimera, Human, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. »
The purity of Ephrin-B2/EFNB2 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »
Ephrin-B2/EFNB2 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Ephrin-B2/EFNB2 hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human EPHB2, His Tag with an affinity constant of 82.97 pM as determined in SPR assay (Biacore T200). »
| Target Background | Ephrin-B2 controls platelet-derived growth factor receptor β (PDGFRβ) distribution in the VSMC plasma membrane, endocytosis, and signaling in a fashion that is highly distinct from its role in the endothelium. Ephrin-B2 is an important regulator of PDGFRβ endocytosis and thereby acts as a molecular switch controlling the downstream signaling activity of this receptor in mural cells. |
| Synonyms | Ephrin B2; EPLG5; HTKL; LERK5; EFNB2 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
