| ￥2100 | |
| Z05246-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 54.2 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Immobilized EpCAM/TROP1 hFc Chimera, Human, hFc Tag at 2 μg/ml(100 μl/well) on the plate. Dose response curve for Biotinylated Anti-EpCAM Antibody, hFc Tag with the EC50 of 37.0ng/ml determined by ELISA. »
The purity of EpCAM/TROP1 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »
EpCAM/TROP1 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.. »
EpCAM/TROP1 hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human AGR-2, His Tag with an affinity constant of 6.91 μM as determined in SPR assay (Biacore T200). »
| Target Background | Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain. |
| Synonyms | CD326; EGP; EGP-2; EGP314; EGP40; EpCAM; ACSTD1; DIAR5; gp40; HNPCC8; MOC31; TACST-1; TACSTD1; TROP1; ESA; KSA; MIC18; MK1 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
