| ￥2200 | |
| Z05224-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 52.8 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 70-100 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Immobilized Biotinylated Human CD155, His Avi Tag at 2 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for DNAM-1/CD226 hFc Chimera, Human, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA. »
The purity of DNAM-1/CD226 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »
DNAM-1/CD226 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
DNAM-1/CD226 hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque CD155, His Tag with an affinity constant of 0.234 μM as determined in SPR assay (Biacore T200). »
| Target Background | DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2. |
| Synonyms | CD226 molecule; CD226; DNAM1; PTA1; TLiSA1 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
