| ￥3000 | |
| Z05062-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 53.4 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Loaded B7-H6/NCR3LG1 hFc Chimera, Human, hFc Tag on ProA-Biosensor, can bind Human NKp30, His Tag with an affinity constant of 0.51 µM as determined in BLI assay (Gator). »
Immobilized Human NKp30, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for B7-H6/NCR3LG1 hFc Chimera, Human, hFc Tag with the EC50 of 55.9ng/ml determined by ELISA. »
The purity of B7-H6/NCR3LG1 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »
B7-H6/NCR3LG1 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
B7-H6/NCR3LG1 hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human NKp30, His Tag with an affinity constant of 0.292 μM as determined in SPR assay (Biacore T200). »
| Target Background | B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins. which is a ligand for the NK cell activating receptor NKp30, was targeted to create a CAR that targets multiple tumor types. B7H6 is expressed on various primary human tumors, including leukemia, lymphoma and gastrointestinal stromal tumors, but it is not constitutively expressed on normal tissues. |
| Synonyms | B7H6; B7-H6; DKFZp686I21167; DKFZp686O24166; NCR3LG1; B7 Homolog 6 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
