| ￥3000 | |
| Z05056-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 53.4 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 75-100 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Immobilized Human ICOS, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for B7-H2/ICOSLG hFc Chimera, Human, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA. »
The purity of B7-H2/ICOSLG hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »
B7-H2/ICOSLG hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
B7-H2/ICOSLG hFc Chimera, Human captured on Protein A chip, can bind Human ICOS (C136S,C137S) , His Tag with an affinity constant of 40.2nM as determined in a SPR assay (Biacore T200). »
| Target Background | B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is a ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. |
| Synonyms | CD275; B7H2; B7-H2; B7RP-1; GL50; ICOSL; ICOS-L; ICOSLG; B7RP1; LICOS; ICOS ligand; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
