| ￥2600 | |
| Z05052-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 50.5 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Immobilized Human CTLA-4, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for B7-1/CD80 hFc Chimera, Human, hFc Tag with the EC50 of 21.6ng/ml determined by ELISA. »
The purity of B7-1/CD80 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »
B7-1/CD80 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
B7-1/CD80 hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human/Cynomolgus/Rhesus macaque CD28, His-Avi Tag with an affinity constant of 43.61 μM as determined in SPR assay (Biacore T200). »
| Target Background | Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4. |
| Synonyms | CD80 molecule; B7; B71; B7-1; BB1; CD28LG1; CD80; B7.1; CD28LG1; LAB7 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
