| ￥3500 | |
| Z04778-100 | |
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| Species | Cynomolgus | ||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 27.7 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 48-68 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Immobilized DNAM-1/CD226, His, Cynomolgus, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 1.79 μg/ml determined by ELISA. »
The purity of DNAM-1/CD226, His, Cynomolgus is greater than 95% as determined by SEC-HPLC. »
DNAM-1/CD226, His, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind DNAM-1/CD226, His, Cynomolgus, His Tag with an affinity constant of 0.82 μM as determined in SPR assay (Biacore T200). »
| Target Background | DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2. |
| Synonyms | CD226 molecule; CD226; DNAM1; PTA1; TLiSA1 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
