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目录产品 » TNF alpha[Biotin], His & Avi, Human
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TNF alpha[Biotin], His & Avi, Human

Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
￥5800
Z04626-100

产品简介
Species Human
Protein Construction
TNF alpha (Val77-Leu233)
Accession # P01375		 His Avi
N-term C-term
Conjugate Biotin
Purity > 90% as determined by Bis­-Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 20.3 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 21-24 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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图例
TNF Alpha[Biotin], His & Avi, Human

Immobilized Human TNFR2, mFc Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for TNF alpha[Biotin], His & Avi, Human, His Avi Tag with the EC50 of 11.5ng/ml determined by ELISA. »

TNF Alpha[Biotin], His & Avi, Human

The purity of TNF alpha[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. »

TNF Alpha[Biotin], His & Avi, Human

TNF alpha[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. »

TNF Alpha[Biotin], His & Avi, Human

Biotinylated TNF alpha[Biotin], His & Avi, Human, His Tag captured on CM5 Chip via Streptavidin can bind Human TNFR2, mFc Tag with an affinity constant of 1.18 nM as determined in SPR assay (Biacore T200). »

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技术背景
Target Background Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Synonyms APC1 protein; Cachectin; DIF; TNF; TNFalpha; TNFATNF; TNFSF1A; TNFSF2; TNFA; TNFα; DIF; TNFSF2;TNF-alpha
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.