澳门大阳城122.ccapp官方下载

目录产品 » Nectin-3/CD113[Biotin], His & Avi, Human
Manual
COAs

Nectin-3/CD113[Biotin], His & Avi, Human

The Nectin family has at least four members (Nectin-1‑4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein.
￥5800
Z04565-100

产品简介
Species Human
Protein Construction
Nectin-3/CD113 (Gly58-Asp400)
Accession # Q9NQS3-1		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE
Endotoxin Level Less than 0.05EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 40.6 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 68-82 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in 20mM PB, 250mM NaCl (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
返回

图例
Nectin-3/CD113[Biotin], His & Avi, Human

Immobilized Biotinylated Nectin-3/CD113[Biotin], His & Avi, Human, His Avi Tag at 5 μg/ml(100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA. »

Nectin-3/CD113[Biotin], His & Avi, Human

The purity of Nectin-3/CD113[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. »

Nectin-3/CD113[Biotin], His & Avi, Human

Nectin-3/CD113[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. »

Nectin-3/CD113[Biotin], His & Avi, Human

Biotinylated Nectin-3/CD113[Biotin], His & Avi, Human, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.13 μM as determined in SPR assay (Biacore T200). »

<
>

技术背景
Target Background The Nectin family has at least four members (Nectin-1‑4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein.
Synonyms CD113; CDw113; FLJ90624; PPR3; PVRL3; PVRR3; PRR3
返回

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.