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Latent TGF beta 1/TGFB1[Biotin], His & Avi, Human

Latent TGF beta 1 cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1. Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex.
￥6600
Z04533-100

产品简介
Species Human
Protein Construction
Avi His Latent TGF beta 1/TGFB1 [Leu30-Ser390 (C33S)]
Accession # P01137
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 44.2 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 40-50 kDa (LAP) / 13-15 kDa (Mature TGF beta 1) based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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图例
Latent TGF Beta 1/TGFB1[Biotin], His & Avi, Human

Immobilized Human ITGAV&ITGB6, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Latent TGF beta 1/TGFB1[Biotin], His & Avi, Human, His-Avi Tag with the EC50 of 48.7ng/ml determined by ELISA. »

Latent TGF Beta 1/TGFB1[Biotin], His & Avi, Human

Immobilized Human ITGAV&ITGB8, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Latent TGF beta 1/TGFB1[Biotin], His & Avi, Human, His Tag with the EC50 of 0.85 μg/ml determined by ELISA. »

Latent TGF Beta 1/TGFB1[Biotin], His & Avi, Human

The purity of Latent TGF beta 1/TGFB1[Biotin], His & Avi, Human 1 is greater than 90% as determined by SEC-HPLC. »

Latent TGF Beta 1/TGFB1[Biotin], His & Avi, Human

Latent TGF beta 1/TGFB1[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

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技术背景
Target Background Latent TGF beta 1 cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1. Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex.
Synonyms CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1; TGF β1; TGFβ; TGF-β-1
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.