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CD123 mRNA (Cap1, m1Ψ)

CD123 is the alpha chain of the interleukin 3 receptor (IL-3R). It is a molecule found on cells which helps transmit the signal of interleukin-3, a soluble cytokine important in the immune system.
¥1500
RP-A00058-0.2

Description

CD123 is the alpha chain of the interleukin 3 receptor (IL-3R). It is a molecule found on cells which helps transmit the signal of interleukin-3, a soluble cytokine important in the immune system.  
 
This mRNA features: 
- A Cap 1 structure with high capping efficiency 
- Codon optimized using GenScript X5 algorithm to enhance expression efficiency 
- 100% substitution with N1-methyl-pseudouridine (m1Ψ) for improved protein expression and reduced innate immune response 
- GenScript’s patented UTR to enhance translation 
- A 100A poly(A) tail to mimic natural mature mRNA

Form Liquid
Concentration 1mg/mL
Full mRNA length 1402 nt
Full mRNA Molecular Weight 453088 Da
Storage buffer 1mM Sodium citrate, pH 6.5
Storage condition Store at -20°C for short term (<3 months), store at -80°C for long term.
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Appearance Clear and free of foreign particles
RNA Length Expected size band detected
RNA Content Target ± 5%
Integrity ≥ 75%
OD260/OD280 1.70 ~ 2.30
Capping Efficiency ≥ 90%
Endotoxin < 10 EU/mg
pH Target ± 0.5
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Transfect 0.5μg of CD123 mRNA into one well of cells in a 24-well plate with Lipofectamine™ MessengerMAX™ (or equivalent). 24-48 hours after transfection, detect the expression of CD123 protein using flow cytometry with staining by BD Pharmingen™ APC Mouse Anti-Human CD123 (Catalog No:560087).
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CD123 MRNA (Cap1, M1Ψ)

Expression of CD123 mRNA in A549 cells. GenSmart, X4, and X5 represent GenScript mRNA codon optimized using different algorithms. »

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For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.