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Adenine base editors (ABEs) system is constructed by fusing an artificially evolved adenine deaminase to a mutated Cas9 that is a single-strand DNA nickase, can precisely and permanently convert A·T to G·C with the guidance of a target-specific guide RNA (gRNA) without creating a double-strand DNA break and without requiring an exogenous DNA repair donor. This mRNA is human codon optimized ABE8e, with sequence originally from Richter, M.F. et al Nature Biotechnology volume 38, pages883–891 (2020). |
| Form | Liquid |
| Concentration | 1mg/mL |
| Full mRNA length | 5084 nt |
| Full mRNA Molecular Weight | 1656299 Da |
| Storage buffer | 1mM Sodium citrate, pH 6.5 |
| Storage condition | Store at -20°C for short term (<3 months), store at -80°C for long term. |
| Appearance | Clear and free of foreign particles |
| RNA Length | Expected size band detected |
| RNA Content | Target ± 5% |
| Integrity | ≥ 75% |
| OD260/OD280 | 1.70 ~ 2.30 |
| Capping Efficiency | ≥ 90% |
| Endotoxin | < 10 EU/mg |
| pH | Target ± 0.5 |
| Transfect 0.5 µg of ABE8e mRNA and sgRNA (at a 2:1 mass ratio) into 2×10⁵ HEK293T cells (through LNP encapsulation, electroporation, or transfection reagent). 48 hours after transfection, extract genomic DNA, and amplify the target region by PCR. Analyze the PCR products through Sanger sequencing and EditR tool. |
ABE8e mRNA and EXM1 sgRNA encapsulated with either SM102 or SM102-Mod LNPs were able to mediate efficient base editing at A3, A5, and A6 sites. However, bystander editing was observed at the A7 site. The editing efficiency of the SM102-Mod LNP group was higher than that of the SM102 LNP group. »
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
