Description
The GenLNP-A01-FLuc mRNA (m1Ψ) is a LNP formulation loaded with firefly luciferase mRNA (m1Ψ) prepared using generic LNP formulation with main ionizable lipid of ALC0315.
The payload Fluc mRNA (m1Ψ) has all the U bases 100% modified with N1-methyl-pseudouridine (m1Ψ). F-Luc mRNA can translate into F-Luc and emit bioluminescence in the presence of its substrate, Luciferin. Fluc mRNA is a commonly used fluorescence reporter sequence in mRNA therapeutics and delivery system development projects.
This formulation is a good control for testing if ALC0315 LNP formulation is a good potential formulation for your mRNA product under research in your in vitro and in vivo experiment model. |
| Form |
Liquid |
| Concentration |
0.15mg/mL |
| Full mRNA length |
1909nt |
| Full mRNA Molecular Weight |
622225 |
| Storage buffer |
Tris-HCl/Sucrose |
| Storage condition |
Store at -80°C for long term. Avoid freeze thaw, only thaw once before use. |
| Appearance |
Clear and free of foreign particles |
| Encapsulation Efficiency |
> 85% |
| Endotoxin |
< 4 EU/ml |
| Particle Size |
50-100 nm |
| pH |
7.4 ± 0.5 |
| Polydispersity Index |
< 0.2 |
| Zeta Potential |
± 15.0 mV |
1. LNP can be added directly to cells incubated in complete media
2. Cells grown and transfected in complete media (DMEM +10% FBS) has higher expression than reduced serum medium
3. For suspension cell lines, we recommend using low fluorescent background media such as Flourobrite® with 10% FBS, 1X GlutaMax® and 1X sodium pyruvate
4. For adherent cell line, we recommend replace complete media with Opti-mem right before reading the cell eGFP expression
5. For higher expression, we recommend incubate the LNP with FBS at 1:2 volume ratio (LNP:FBS) for 30 minutes at 37C before transfecting |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
