Description
The EGFP self-amplifying RNA (saRNA) expresses an enhanced green fluorescent protein, with the open reading frame sequence originally from the jellyfish, Aequorea Victoria. EGFP saRNA is a commonly used fluorescence reporter sequence in mRNA therapeutics and delivery system development projects. The expressed protein has an excitation wavelength of 488nm, emission at 507nm.
This saRNA features:
- A Cap1-AU structure with high capping efficiency
- 100% substitution with 5-methylcytidine (m5C) for improved protein expression and reduced innate immune response
- Constructed using GenScript’s patented saRNA Vector 1
- A 68A poly(A) tail to mimic natural mature mRNA |
| Form |
Liquid |
| Concentration |
1mg/mL |
| Full mRNA length |
9891nt |
| Full mRNA Molecular Weight |
3441479 |
| Storage buffer |
1mM Sodium citrate, pH6.5 |
| Storage condition |
Store at -20°C for short term (<3 months), store at -80°C for long term. |
| Appearance |
Clear and free of foreign particles |
| RNA Length |
Expected size band detected |
| RNA Content |
Target ± 5% |
| Integrity |
≥ 70% |
| OD260/OD280 |
1.70 ~ 2.30 |
| Capping Efficiency |
≥ 90% |
| Endotoxin |
< 10 EU/mg |
| pH |
Target ± 0.5 |
|
Transfect 0.01-0.05 µg of saRNA with 0.5 µL of Lipofectamine™ 2000 (or equivalent reagent) per well in a 96-well plate, following the manufacturer’s protocol. Analyze the expression 16-48 hours post-transfection using a plate reader, flow cytometer, fluorescence microscope, or confocal microscope. For equipment settings, we recommend: 485 nm excitation, 535 nm emission. |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
