Description
The GenLNP-S02-EGFP mRNA (m1Ψ) is a TriGalNac ligand decorated LNP formulation with main ionizable lipid of SM102.
The payload eGFP mRNA (m1Ψ) has all the U bases 100% modified with N1-methyl-pseudouridine (m1Ψ). This LNP formulation showed enhanced delivery of eGFP mRNA to hepatocyte after IV injection into mice.. |
| Form |
Liquid |
| Concentration |
0.15mg/mL |
| Full mRNA length |
980nt |
| Full mRNA Molecular Weight |
319792 |
| Storage buffer |
PBS/Sucrose |
| Storage condition |
Store at -80°C for long term. Avoid freeze thaw, only thaw once before use. |
| Appearance |
Clear and free of foreign particles |
| Encapsulation Efficiency |
> 85% |
| Endotoxin |
< 4 EU/ml |
| Particle Size |
65-125 nm |
| pH |
7.4 ± 0.5 |
| Polydispersity Index |
< 0.2 |
| Zeta Potential |
± 15.0 mV |
1. LNP can be added directly to cells incubated in complete media
2. Cells grown and transfected in complete media (DMEM +10% FBS) has higher expression than reduced serum medium
3. For suspension cell lines, we recommend using low fluorescent background media such as Flourobrite® with 10% FBS, 1X GlutaMax® and 1X sodium pyruvate
4. For adherent cell line, we recommend replace complete media with Opti-mem right before reading the cell eGFP expression
5. For higher expression, we recommend incubate the LNP with FBS at 1:2 volume ratio (LNP:FBS) for 30 minutes at 37C before transfecting |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
