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| M00771 | |
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| Product Description | Recombinant CHO-K1 cells stably express Angiotensin-converting enzyme 2 (ACE2) on the cell surface. The surface expression of ACE2 is validated by FACS analysis. This stable cell line product is designed for cell-based binding assays that measure the binding affinity and stability of antibody based biologics binding with ACE2. |
| Culture Properties | Adherent |
| Stability | Stability: Stable through more than 20 passages with no significant changes in assay performance or expression profile. |
| Size | Two vials of frozen cells (>1×106 per vial in 1 mL) |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium | F-12K (Kaighn’s), 10% FBS, 250 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies) |
| Complete Growth Medium | F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies) |
| Freeze Medium-DATA | 95% complete growth medium, 5% DMSO (Cat. No. D2650, Sigma) |
Figure 1. FACS analysis of cell surface expression of ACE2 on CHO-K1 cells. CHO-K1/ACE2 cells (green) and negative control CHO-K1 cells (blue) were stained with SARS-CoV-2 Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript).
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Figure 2. FACS analysis of cell surface expression of ACE2 on CHO-K1 cells of different passages. The CHO-K1/ACE2 cells and the negative control CHO-K1 cells were stained with Spike protein (RBD, His Tag) (Cat. No. Z03479, GenScript). P6, P11, P16 and P20 are cell passage numbers. »
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
