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目录产品 » 稳定细胞系 » Human Recombinant CD16A 158F Stable Cell Line
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CHO-K1/CD16A 158F Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human CD16A and Fc epsilon receptor Ig (FcER1G) on the surface. The surface expression of CD16A is validated by FACS analysis.
询价
M00586

产品简介
Product Description Recombinant CHO-K1 cells stably overexpress human CD16A and Fc epsilon receptor Ig (FcER1G) on the surface. The surface expression of CD16A is validated by FACS analysis.
Culture Properties Adherent.
Stability Stability: Stable through more than 15 passages without significant changes in assay performance or expression profile.
Size Two vials of frozen cells (>1×106 per vial in 1 mL)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
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培养条件
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 4 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies), 100 μg/ml Hygromycin B (Cat. No.10687010, Life Technologies)
Complete Growth Medium Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies)
Freeze Medium-DATA 95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma).
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图例
CHO-K1/CD16A 158F Stable Cell Line

Figure 1. FACS analysis of cell surface expression of CD16A 158F on CHO-K1/CD16A 158F cells. The CHO-K1/CD16A 158F cells (bule) and the negative control CHO-K1 cells (red) were probed using PerCP/Cy5.5 anti-human CD16 (Cat. No. 302028, Biolegend).

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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.