一步实现，无需人工干预，确保结果高重复性
满足大体积纯化应用需求
蛋白产量可达500ug-240mg/样本
仅需30分钟完成12个样本纯化
| 产品类型 | 产品编号 | 产品名称 | 数量 | 规格 |
|---|---|---|---|---|
| 设备 | D00045 | AmMag™ Quatro ProAb 1300 | 1 Unit | 询价 |
| 耗材 | D00065 | AmMag™ Quatro 100mL Centrifuge Tube Rack | 1 pcs/box | 询价 |
| D00068 | AmMag™ Quatro 12-Channel Large Magnetic Rod | 1 pcs/box | 询价 | |
| D00064 | AmMag™ Quatro 50mL Centrifuge Tube Rack | 1 pcs/box | 询价 | |
| D00069 | AmMag™ Quatro 12-Channel Small Magnetic Rod | 1 pcs/box | 询价 | |
| D00066 | AmMag™ Quatro 100mL Centrifuge Tube Magnetic Rack | 1 pcs/box | 询价 | |
| D00080 | AmMag™ Quatro 25mL Centrifuge Tube Rack | 1 pcs/box | 询价 | |
| D00061 | AmMag™ 100 mL centrifuge tube | 150 pcs/box | 询价 | |
| D00060 | AmMag™ Four-slot magnetic rod sleeve | 30 pcs/box | 询价 | |
| D00071 | AmMag™ Quatro Four-slot Small Magnetic Rod Sleeve | 30 pcs/box | 询价 | |
| D00072 | AmMag™ Quatro 50mL Round-bottom Centrifuge Tube | 50 pcs/pack 16packs/box |
询价 | |
| D00081 | AmMag™ Quatro 25mL round-bottom centrifuge tube | 50 pcs/pack 16packs/box |
询价 | |
| D00074 | AmMag™ Quatro Reagent Bottle And Tubing Kit | 6 EA/1 Unit | 询价 |
| AmMag™ Quatro 1300 | |
|---|---|
| 样本类型 | 细胞发酵液 |
| 样本最大上样体积 | 65 mL |
| 样本最小上样体积 | 3 mL |
| 样本管 / 反应管 | 25 mL, 50 mL, 100 mL离心管 |
| 仪器通量 | 12个 |
| 试剂储存条件 | 室温 |
| 提取方式 | 磁珠法 |
| 试用 | 申请试用 >> |
回收率一致
纯度一致
活性一致
图1：AmMag™ Quatro 1300与手动磁珠纯化方法性能一致
取30 mL过表达Anti-E2 hIgG 的CHO细胞发酵液, 采用BLI方法测定抗体滴度为923.3 μg/mL。取用2 mL太阳成集团122ccProtein A Magnetic Beads (Cat# L00695)，分别用AmMag™ Quatro 1300全自动蛋白/抗体磁珠纯化系统和手动磁珠法进行样本纯化。随后，对纯化后的抗体进行收率、纯度（SEC-HPLC）、结合活性检测。
结果表明：两种纯化方式的回收率、纯度（ 均>95%）一致，活性均与阳性对照样品相当。
PC: 阳性对照（介质纯化样品）； GS-1300: 用GenScript AmMag™ Quatro 1300纯化的样本；GS-手动：用GenScript磁珠(Cat# L00695)手动法纯化的样本。
回收率均一
纯度均一
活性均一
图2：AmMag™ Quatro 1300蛋白纯化系统重复性高
在AmMag™ Quatro 1300系统上，使用AmMag™ Quatro Elite Protein A Magnetic Beads, 2G (Cat#L01074)磁珠，对过表达Anti-N protein hIgG 的CHO细胞上清进行纯化（30 mL/样本），12通道测试，对纯化后的抗体进行回收率、纯度和活性检测。
结果表明：相同样本情况下，仪器12通道之间蛋白回收率、纯度和活性重复性高，证明AmMag™ Quatro 1300蛋白纯化系统重复性高。
PC: 阳性对照（介质纯化样本）； GS-1300: 用GenScript AmMag™ Quatro 1300纯化的样本；GS-手动：用GenScript磁珠（Cat#L01074）手动法纯化的样本。
样品信息
抗体类型：hIgG；表达系统：CHO细胞表达；样本体积：30 mL；Titer：样本1--923 μg/mL；样本2--114 μg/mL；样本3--23 μg/mL；
高活性
样本1
样本2
样本3
回收率均一
纯度均一
结果显示: AmMag™ Quatro 1300 可以用来纯化不同表达量的样本。
| 亲和类型 | 产品编号 | 产品名称 | 结合载量 |
|---|---|---|---|
|
Protein A beads
|
L00273 | Protein A MagBeads | >10 mg Human IgG/mL settled beads |
| L00695 | AmMag™ Protein A Magnetic Beads | >40 mg Human IgG/mL settled beads | |
| L01071New | AmMag™ Quatro Elite Protein A Magnetic Beads | >40 mg Human IgG/mL settled beads | |
| L01074New | AmMag™ Quatro Elite Protein A Magnetic Beads - 2G | >35 mg Human IgG/mL settled beads | |
|
Protein A/G beads
|
L00894 | Protein A/G Magbeads MX | >30 mg Human IgG/mL settled beads |
| L00277 | Protein A/G MagBeads | >10 mg Human IgG/mL settled beads | |
|
Protein G beads
|
L00274 | Protein G MagBeads | >10 mg Human IgG/mL settled beads |
| L00673 | Protein G MagBeads MX | >30 mg Human IgG/mL settled beads | |
|
Nickel Beads
|
L00295 | Ni-charged MagBeads | >40mg His-tagged Protein/mL settled beads |
| L00776 | AmMag™ Ni Magnetic beads | >20mg His-tagged Protein/mL settled beads | |
|
GST Beads
|
L00895 | Glutathione MagBeads | >20mg GST-tagged Protein/mL settled beads |
