| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | HOXB4 mutants with partially reintroduced PRD (Proline-Rich-Domain) region were obtained by synthesis from GenScript®. Hoxa9-wt cDNA (NM_152739.3) was subcloned into MSCV/EGFP, while Hoxa9-Pro-in423, Hoxa9-Pro-in813, Hoxa9-scramble (random amino acids (aa) sequence), and Hoxa9 Pro-in False (same amino acids in random order) mutants were obtained by gene synthesis from GenScript® and subcloned into the same vector. | Get A Quote |
There is high interest in understanding the mechanisms that drive self-renewal of stem cells. HOXB4 is one of the few transcription factors that can amplify long-term repopulating hematopoietic stem cells in a controlled way. Here we show in mice that this characteristic of HOXB4 depends on a proline-rich sequence near the N terminus， which is unique among HOX genes and highly conserved in higher mammals. Deletion of this domain substantially enhanced the oncogenicity of HOXB4， inducing acute leukemia in mice. Conversely， insertion of the domain into Hoxa9 impaired leukemogenicity of this homeobox gene. These results indicate that proline-rich stretches attenuate the potential of stem cell active homeobox... More
