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Evaluation of Gaussia luciferase and foot-and-mouth disease virus 2A translational interrupter chimeras as polycistronic reporters for transgene expression.

BMC Biotechnol.. 2017-06; 
PucketteMichael, BurrageThomas, NeilanJohn G, Rasmusse
Products/Services Used Details Operation
Gene Synthesis … Creation of Δ1D2A-GLuc/SGLuc chimeras. For construction of Δ1D2A-GLuc/SGLuc chimeras, nucleotide sequence encoding the Δ1D2A-SGLuc sequence was synthesized by GenScript and cloned into the pUC57kan vector … Get A Quote

摘要

The Gaussia princeps luciferase is used as a stand-alone reporter of transgene expression for in vitro and in vivo expression systems due to the rapid and easy monitoring of luciferase activity. We sought to simultaneously quantitate production of other recombinant proteins by transcriptionally linking the Gaussia princeps luciferase gene to other genes of interest through the foot-and-mouth disease virus 2A translational interrupter sequence.

关键词

2A,Bicistronic,Biomarker,Foot-and-mouth disease virus,Gaussia luciferase,Polycistronic,Virus-like parti