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Overexpression of TAT-PTD-diapause hormone fusion protein in tobacco and its effect on the larval development of Helicoverpa armigera (Lepidoptera: Noctuidae)

Pest Manag. Sci.. 2017-06; 
ZhouZhou, LiYongli, YuanChunyan, DoucetDaniel, ZhangYongan, QuLiang
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Gene Synthesis … 2 MATERIALS AND METHODS. 2.1 Expression vector construction. The TAT‐caDH sequence (GenBank accession number KJ434109) was chemically synthesised and inserted into the pUC57 plasmid by a commercial service provider (Genscript, Nanjing, China) … Get A Quote

摘要

The diapause hormone (DH) has been shown either to induce or to terminate diapause, depending on the insect species. In a previous study we demonstrated that the DH from Clostera anastomosis (caDH) has biological activity in Helicoverpa armigera, which prompted us to examine the potential growth-inhibiting or antiherbivory effects of the TAT-caDH fusion protein when expressed in transgenic plants.

关键词

Helicoverpa armigera,diapause hormone,fusion protein,transactivator of transcription (