| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | ... Sequences corresponding to the main isoforms of CUPID1 and CUPID2 ( Figure S2) were synthesized by GenScript and inserted into the Nhe1-Not1 or NheI-BamHI sites of pCDNA3.1. For re-establishment of CUPID1 and CUPID2 expression, the following procedures were ... | Get A Quote |
Breast cancer risk is strongly associated with an intergenic region on 11q13. We have previously shown that the strongest risk-associated SNPs fall within a distal enhancer that regulates CCND1. Here, we report that, in addition to regulating CCND1, this enhancer regulates two estrogen-regulated long noncoding RNAs, CUPID1 and CUPID2. We provide evidence that the risk-associated SNPs are associated with reduced chromatin looping between the enhancer and the CUPID1 and CUPID2 bidirectional promoter. We further show that CUPID1 and CUPID2 are predominantly expressed in hormone-receptor-positive breast tumors and play a role in modulating pathway choice for the repair of double-strand breaks. These data reveal a m... More
